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iv Kit Contents and Storage Shipping and Storage The TOPO TA Cloning¤ Kit is shipped on dry ice Each kit contains a box with TOPO TA Cloning¤ reagents Box 1 and a box with TOP10 or TOP10F One Shot competent cells Box 2 Store Box 1 at 20¡C and Box 2 at 80¡C Types of TOPO
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TOPO TA Cloning ¤ Version E 180714 25 Technical Service ¥ Spread 40˚µl of 40 mg/ml X gal on each LB plate and incubate at 37¡C until ready for use
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